Cloning and expression studies of a LFY cDNA from Brassica juncea
نویسندگان
چکیده
A LEAFY cDNA was cloned from the short-day (SD) plant Brassica juncea cv Varuna. (BjLFY) consists of 1261 bp encoding for a protein of 420 amino acids and an estimated isoelectric point of 6.8. The deduced amino acid showed 99% and 86% identity to Arabidopsis thaliana and Brassica oleracea LFY cDNA respectively. The LFY transcript was detected throughout the vegetative and reproductive phase but an increase in transcript level was observed during transition. The earlier induction of BjLFY was observed in early flowering variety of B. juncea as compared to late flowering varieties further proving the critical role LEAFY plays in floral transition.
منابع مشابه
Cloning of dehydrin coding sequences from Brassica juncea and Brassica napus and their low temperature-inducible expression in germinating seeds.
A novel subclass of dehydrin genes, homologous to the Raphanus sativus late embryogenesis-abundant (LEA) protein (RsLEA2) and the Arabidopsis thaliana dehydrin, was isolated from Brassica juncea and Brassica napus, here designated BjDHN1 and BnDHN1, respectively. The cDNA of BjDHN1 and BnDHN1 genes share 100% nucleotide identity. The encoded protein is predicted to consist of 183 amino acid res...
متن کاملCloning and characterization of diacylglycerol acyltransferase (DGAT) cDNA sequence from Brassica juncea cv
Diacylgycerol acyltransferase (DGAT: EC 2.3.1.20) is the only enzyme in the Kennedy pathway that is exclusively committed to the synthesis of storage oil in plants. In this study, cloning and characterization of DGAT gene sequence from Brassica juncea cv Pusa bold, an important oil seed crop of India is reported. A partial gene sequence of 2003 bp was PCR amplified and cloned from B. juncea. Se...
متن کاملCloning of acyl-acyl carrier protein (ACP) thioesterase gene from Brassica juncea.
Acyl-acyl carrier protein (ACP) thioesterase is the chain-length-determining enzyme in de novo biosynthesis of plant fatty acids. For cloning the gene encoding acyl-ACP thioesterase from Brassica juncea, genomic DNA was used as a template to amplify a 0.7 kb thioesterase fragment in a PCR with the primers designed from the known sequences available in the GenBank. This 0.7 kb fragment was used ...
متن کاملCloning and Expression of Human Gamma-Interferon cDNA in E. coli
Prior to the production of human gamma interferon using recombinant DNA technology, it had been producedmainly upon mitogenic induction of lymphocytes in very low amounts, which evidently hamperedits characterization and its medical applications. The recombinant gamma interferons produced in largerquantities in prokaryotic systems retain their biological activities, and can be...
متن کاملHeavy metal toxicity: Effect on plant growth, biochemical parameters and metal accumulation by Brassica juncea L.
Plant growth, pigment concentration, biochemical parameters and uptake of heavy metals were determined for Brassica juncea L. in response to cadmium and lead stress. The plant exhibited a decline in growth, chlorophyll content and carotenoids with Cd and Pb but Cd was found to be more detrimental than Pb treatment in B. juncea. The protein content was decreased by Cd (900 μM) to 95% and 44% by ...
متن کامل